search for ureaplasma urealyticum in the semen of infertile men

The potential role of ureaplasma urealyticum in human infertility makes it desirable to screen barren couples for this infection. Semen specimens from 30 patients were studied. The presence of Ureaplasma was looked for by use of Mycofast kit [liquid technique]. U. Urealyticum was found in 6 [20%] patients in high numbers 10[5]/ml. These results were correlated with some semen parameters [% of abnormal forms and motility]. Infertile patients with Urealplasma had an over-all decrease in semen quality, those lacking ureaplasma also had this poor semen quality with few exception. These data provide some evidence for the association of ureaplasma genital infection and male infertility. So I recommend culture of the semen for ureaplasma as part of investigation of infertility

Latex particle aggultination tests as an adjunct to the diagnosis of bacterial meningitis

A prospective study using a latex particle agglutination test for the detection of bacterial antigens in CSF has been carried out in 90 patients with meningitis. The antigens sought were those of Str. pneumoniae, H. influenzae b, N. meningitidis groups A, C, Y, W, 135, N. meningitidis B, E. coli, Kl. and Str. group B. Eighty-five patients had proven bacterial meningitis and the rest had aseptic meningitis. The bacteria causing meningitis included N. meningitidis [group B in four and groups ACYW 135 in 56], Str. pneumoniae [20 patients] and H. influenzae b [five patients]. The sensitivity and specificity of the tests were as good as those reported from developed countries. With both technical feasibility and good sensitivity and specificity, the Wellcogen agglutination tests seem to be useful and reliable for the rapid diagnosis of bacterial meningitis, especially in situations where pre-hospital treatment with antibiotics are possible

Antibiotic susceptibility patterns and some potential virulence factors of clinical isolates of serratia marcescens

During 8-month period, 20 strains of S. marcescens were isolated predominantly from hospitalized patients. These isolates were tested for the occurrence of nine potential virulence determinants. DNase, lecithinase, lipase, proteolytic activity and motility were demonstrated in 100% of isolates, hemolytic activity in 40%, urease activity in 30% and pigmentation in 20%. Generally, these strains were sensitive to many antibiotics, mainly aminoglycosides, third generation cephalosporins and the newer quinolines and resistant to ampicillin, cephalothin, cefamandole and tetracyclines

Haemorrhagic colitis and escherichia coli. 0157: H7

This work included 71 patients with bloody diarrhea aiming to determine the role of Escherichia coli 0157: H7 [E. coli 0157: H7] that has recently associated with hemorrhagic colitis [HC] in all age group and with hemolytic uremic syndrome [HUS] in children. All patients were from villages near by Rositta and Kafr-El- Dawar. This study was carried out during the period from May 1989 to October 1990, where outbreaks and many sporadic cases have been reported. Thorough clinical and laboratory investigations were done. Bacteriological study of the stool samples revealed heavy growth of E. Coli with no other definite bacterial pathogen in 66 cases, E. coli 0157: H7 was identified in only two of these 66 samples, one from case of HC and the second from HUS. The other 64 E. coli have not been serotyped. Klebsiella oxytoca was the pathogen in 3 cases whereas campylobacter in only two cases. E. coli 0157: H7 isolates were sensitive to aminoglycosides and tetracyclines but resistant to ampicillin cephalothin, chloramphenicol, and trimethoprim- sulfamethoxazole. Also, these serotypes were not invasive by standard tests. From this study, it was concluded that E. coli 0157: H7 is a rare serotype in this locality

Clinical, bacteriological and mycological study for otitis externa

Otitis externa is a localized inflammatory skin disorder affecting the external auditory canal and pinna. Many etiological factors are known diseases as seborrheic dermatitis and general disease as diabetes, local factors as scratching, and excessive sweating might predispose to the disease. External climatic condition as high temperature and humidity might share in the development of the disease

Nasal carriage of staphylococcus aureus among diabetic patients

Nasal carriage of S. aureus was studied in 150 diabetic patients [50 adults IDDM, 50 adults NIDDM and 50 diabetic children] and 150 non-diabetic persons [50 children and 100 adults]. The carriage rate of S. aureus was significantly higher in diabetic children [80%] than in normal children [30%], in adults the carriage rate was higher in IDDM to be 35%. There was a marked drop in the carriage rate of the S. aureus after topical use of gentamicin cream [52.6%]

Identification of the microbial flora of the normal and diseased vagina in pregnant and non pregnant women

The vaginal flora was studied in 100 sexually active women [mean age 30 years, range 20-38]. 50 were pregnant and 50 non- pregnant. Each group included 30 women complaining of heavy vaginal discharge and 20 asymptomatic. High vaginal swabs were taken and subjected to full bacteriological study. Nearly all women either symptomatic or not harbor pathogenic or potentially pathogenic microorganisms. The predominant microorganisms were G. vaginalis, Lactobacilli, Diphtheroid, Micrococci and Staph. epidermidosis. It was concluded that the vaginal flora was similar regardless of vaginitis or amount of vaginal discharge and the major difference was more prevalence of known vaginal pathogens [T. vaginalis and C. albicans] among women with vaginitis. No attempt was made to seek viruses, chlamydia and mycoplasma

bacteriology of secretory otitis media [SOM] in children

Aerobic and anaerobic bacteriological cultures were taken from middle ear effusions and the respective nasopharynx of 20 patients with secretory otitis media [SOM]. 70% of the effusions and 80% of the nasopharynx yielded positive culture for aerobic bacteria. The most frequent species in effusion samples were Staph. epidermidis and H. influenzae, while N. catarrhalis, H. influenzae and Staph. epidermidis were the most frequent species in the nasopharynx. Anaerobes were not isolated. In conclusion, it appears that aerobic bacteria may play a role in the pathogenesis of SOM and it can be assumed that the nasopharyngeal colonization may be a substantial causal factor of middle ear inflammation

Ophthalmia neonatorum in Tanta prevalence of chlamydia trachomatis

During nine months, 112 infants 1-4 weeks old with purulent ocular discharge and 40 normal infants were studied opthalmologicaly and microbiologicaly for bacteria and chlamydia aetiology. Infants with conjunctivitis were divided into two groups : group [A] had no treatment and group [B] treated before with no response. Pathogenic bacteria were cultured in 49.1% of all cases. Chlamydia was found in 9.6% in group [A] and in 38% in group [B]. In other words Chlamydial ophthalmia neonatorum is not uncommon in Tanta